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The bound protein was eluted in binding buffer supplemented with 300 mM NaCl at a flow rate of 3 ml/min and dialyzed against 20 mM sodium phosphate, pH 6.0, with 50 mM NaCl

Dimethyl sulfoxide (DMSO, vehicle) (#D2650), Vincristine Sulfate (Vin) (#V8388), N-acetyl-cysteine (#A15409) and retinoic acid (#R2625) were from Merck

The exact steps can vary based on the third-party testing service, but this typically involves performing laboratory tests on the finished supplement and evaluating the methods by which it is produced in a manufacturing facility

However, VIGS has not been used to identify genes that play various roles in abiotic stress tolerance through a high-throughput forward genetics screening